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InnateDB Group ID
|
950
|
|
Short name
|
AGER :: S100A12
|
|
Full name
|
AGER physically interacts with S100A12
|
|
Interaction type
|
physical interaction
|
|
Participant 1
|
|
Molecule:
|
IDBG-80463
|
|
Molecule type:
|
protein
|
|
Species:
|
Homo sapiens
|
|
HUGO:
|
AGER
|
|
Full name:
|
advanced glycosylation end product-specific receptor
|
|
Direction:
|
input
|
|
Biological role:
|
unspecified role
|
|
Participant 2
|
|
Molecule:
|
IDBG-102649
|
|
Molecule type:
|
protein
|
|
Species:
|
Homo sapiens
|
|
HUGO:
|
S100A12
|
|
Full name:
|
S100 calcium binding protein A12
|
|
Direction:
|
input
|
|
Biological role:
|
unspecified role
|
|
InnateDB ID
|
IDB-187437.2
|
|
Last Modified
|
2010-07-04
|
|
Review status
|
reviewed
[Report errors or provide feedback]
|
|
Source database
|
INNATEDB
|
|
Source database ID
|
IDB-187437
|
|
PMID
|
19727205
|
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Interaction detection method
|
solid phase assay |
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Host system
|
invitro |
|
Host organism
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|
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Cell status
|
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Cell line
|
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Cell type
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Tissue type
|
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Comment
|
Recombinant AGER was added to a flat-bottom plate which was coated overnight with various concentrations ( 2, 10 and 50 ug/ml) of recombinant S100A12 (Fig 5). The Kd value of AGER for S100A12 was determined to be 1.39 uM and the interaction was Ca+2 and Zn+2 but not Mg+2 dependent. This implies that Ca+2 and Zn+2 enhances the conformation of S100A12 required for the optimal binding to AGER. |
|
Participants
|
|
Participant Identification Method |
Experimental Role |
Participant Accession |
| 1) AGER |
predetermined participant |
prey |
|
| 2) S100A12 |
predetermined participant |
bait |
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|
|
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Version 5.4