InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: GRK4

Summary

InnateDB Protein

IDBP-233961.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

GRK4

Protein Name

G protein-coupled receptor kinase 4

Synonyms

GPRK2L; GPRK4; GRK4a; IT11;

Species

Homo sapiens

Ensembl Protein

ENSP00000381129

InnateDB Gene

IDBG-7807 (GRK4)

Protein Structure

UniProt Annotation

Function

Specifically phosphorylates the activated forms of G protein-coupled receptors. GRK4-alpha can phosphorylate rhodopsin and its activity is inhibited by calmodulin; the other three isoforms do not phosphorylate rhodopsin and do not interact with calmodulin. GRK4-alpha and GRK4-gamma phosphorylate DRD3. Phosphorylates ADRB2. {ECO:0000269PubMed:19520868, ECO:0000269PubMed:8626439}.

Subcellular Localization

Cytoplasm. Cytoplasm, cell cortex. Note=Both at the cell surface and dispersed in the cytoplasm under basal conditions. Receptor stimulation results in the internalization of GRK4 to the perinuclear area, where colocalization with DRD3 is observed strongly at 5 and 15 minutes. DRD3 and GRK4 colocalize in lipid rafts of renal proximal tubule cells.

Disease Associations

Tissue Specificity

Isoform 1, isoform 2, isoform 3, and isoform 4 are expressed in testis. Isoform 4 is expressed in myometrium. {ECO:0000269PubMed:16636192, ECO:0000269PubMed:8626439}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 11 experimentally validated interaction(s) in this database.

Experimentally validated
Total	11 [view]
Protein-Protein	7 [view]
Protein-DNA	4 [view]
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Gene Ontology

Molecular Function

Accession	GO Term
GO:0004672	protein kinase activity
GO:0004674	protein serine/threonine kinase activity
GO:0004703	G-protein coupled receptor kinase activity
GO:0004713	protein tyrosine kinase activity
GO:0005524	ATP binding
GO:0016772	transferase activity, transferring phosphorus-containing groups
GO:0050254	rhodopsin kinase activity

Biological Process

GO:0002029	desensitization of G-protein coupled receptor protein signaling pathway
GO:0002031	G-protein coupled receptor internalization
GO:0006468	protein phosphorylation
GO:0007165	signal transduction
GO:0008277	regulation of G-protein coupled receptor protein signaling pathway
GO:0031623	receptor internalization
GO:0038032	termination of G-protein coupled receptor signaling pathway

Cellular Component

GO:0005829	cytosol
GO:0005938	cell cortex
GO:0030425	dendrite
GO:0043025	neuronal cell body

Protein Structure and Domains

PDB ID

InterPro

IPR000239 GPCR kinase
IPR000342 Regulator of G protein signalling domain
IPR000719 Protein kinase domain
IPR000961 AGC-kinase, C-terminal
IPR001245 Serine-threonine/tyrosine-protein kinase catalytic domain
IPR002290 Serine/threonine/dual specificity protein kinase, catalytic domain
IPR011009 Protein kinase-like domain
IPR016137 Regulator of G protein signalling superfamily
IPR020635 Tyrosine-protein kinase, catalytic domain

PFAM

PF00615
PF00069
PF07714

PRINTS

PR00717
PR01301
PR00109

PIRSF

SMART

SM00133
SM00220
SM00315
SM00219

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt