InnateDB: Systems Biology of the Innate Immune Response

Mus musculus Protein: Blk

Summary

InnateDB Protein

IDBP-172524.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

Blk

Protein Name

B lymphoid kinase

Synonyms

Species

Mus musculus

Ensembl Protein

ENSMUSP00000014597

InnateDB Gene

IDBG-172522 (Blk)

Protein Structure

UniProt Annotation

Function

Non-receptor tyrosine kinase involved in B-lymphocyte development, differentiation and signaling. B-cell receptor (BCR) signaling requires a tight regulation of several protein tyrosine kinases and phosphatases, and associated coreceptors. Binding of antigen to the B-cell antigen receptor (BCR) triggers signaling that ultimately leads to B-cell activation. Signaling through BLK plays an important role in transmitting signals through surface immunoglobulins and supports the pro-B to pre-B transition, as well as the signaling for growth arrest and apoptosis downstream of B-cell receptor. Specifically binds and phosphorylates CD79A at 'Tyr-188'and 'Tyr-199', as well as CD79B at 'Tyr-196' and 'Tyr- 207'. Phosphorylates also the immunoglobulin G receptor FCGR2. With FYN and LYN, plays an essential role in pre-B-cell receptor (pre-BCR)-mediated NF-kappa-B activation. Contributes also to BTK activation by indirectly stimulating BTK intramolecular autophosphorylation. In pancreatic islets, acts as a modulator of beta-cells function through the up-regulation of PDX1 and NKX6-1 and consequent stimulation of insulin secretion in response to glucose. {ECO:0000269PubMed:12563261, ECO:0000269PubMed:14662906, ECO:0000269PubMed:2404338, ECO:0000269PubMed:7565679, ECO:0000269PubMed:7592958, ECO:0000269PubMed:7608542, ECO:0000269PubMed:7690139, ECO:0000269PubMed:9177269, ECO:0000269PubMed:9636152}.

Subcellular Localization

Cell membrane {ECO:0000250}; Lipid-anchor {ECO:0000250}. Note=Present and active in lipid rafts (By similarity). Membrane location is required for the phosphorylation of CD79A and CD79B. {ECO:0000250, ECO:0000269PubMed:7592958}.

Disease Associations

Tissue Specificity

Specifically expressed in the B-cell lineage. {ECO:0000269PubMed:1537861, ECO:0000269PubMed:2404338}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 4 experimentally validated interaction(s) in this database.
They are also associated with 12 interaction(s) predicted by orthology.

Experimentally validated
Total	4 [view]
Protein-Protein	4 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	12 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0004672	protein kinase activity
GO:0004713	protein tyrosine kinase activity
GO:0004715	non-membrane spanning protein tyrosine kinase activity
GO:0005515	protein binding
GO:0005524	ATP binding
GO:0016772	transferase activity, transferring phosphorus-containing groups

Biological Process

GO:0006468	protein phosphorylation
GO:0018108	peptidyl-tyrosine phosphorylation
GO:0032024	positive regulation of insulin secretion
GO:0050853	B cell receptor signaling pathway

Cellular Component

GO:0005829	cytosol
GO:0005886	plasma membrane

Protein Structure and Domains

PDB ID

MGI:88169

InterPro

IPR000719 Protein kinase domain
IPR000980 SH2 domain
IPR001245 Serine-threonine/tyrosine-protein kinase catalytic domain
IPR001452 SH3 domain
IPR002290 Serine/threonine- /dual specificity protein kinase, catalytic domain
IPR011009 Protein kinase-like domain
IPR011511 Variant SH3 domain
IPR020635 Tyrosine-protein kinase, catalytic domain

PFAM