InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: XRCC3

Summary

InnateDB Protein

IDBP-596941.3

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

XRCC3

Protein Name

X-ray repair complementing defective repair in Chinese hamster cells 3

Synonyms

CMM6;

Species

Homo sapiens

Ensembl Protein

ENSP00000452598

InnateDB Gene

IDBG-22058 (XRCC3)

Protein Structure

UniProt Annotation

Function

Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. Part of the RAD21 paralog protein complex CX3 which acts in the BRCA1-BRCA2- dependent HR pathway. Upon DNA damage, CX3 acts downstream of RAD51 recruitment; the complex binds predominantly to the intersection of the four duplex arms of the Holliday junction (HJ) and to junctions of replication forks. Involved in HJ resolution and thus in processing HR intermediates late in the DNA repair process; the function may be linked to the CX3 complex and seems to involve GEN1 during mitotic cell cycle progression. Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51 and RAD51C. {ECO:0000269PubMed:14716019, ECO:0000269PubMed:20413593, ECO:0000269PubMed:23108668, ECO:0000269PubMed:23149936}.

Subcellular Localization

Nucleus. Cytoplasm. Cytoplasm, perinuclear region. Mitochondrion. Note=Accumulates in discrete nuclear foci prior to DNA damage, and these foci persist throughout the time course of DNA repair.

Disease Associations

Breast cancer (BC) [MIM:114480]: A common malignancy originating from breast epithelial tissue. Breast neoplasms can be distinguished by their histologic pattern. Invasive ductal carcinoma is by far the most common type. Breast cancer is etiologically and genetically heterogeneous. Important genetic factors have been indicated by familial occurrence and bilateral involvement. Mutations at more than one locus can be involved in different families or even in the same case. {ECO:0000269PubMed:12023982}. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.Melanoma, cutaneous malignant 6 (CMM6) [MIM:613972]: A malignant neoplasm of melanocytes, arising de novo or from a pre- existing benign nevus, which occurs most often in the skin but also may involve other sites. {ECO:0000269PubMed:11059748}. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.

Tissue Specificity

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 17 experimentally validated interaction(s) in this database.

Experimentally validated
Total	17 [view]
Protein-Protein	17 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Gene Ontology

Molecular Function

Accession	GO Term
GO:0000400	four-way junction DNA binding
GO:0003677	DNA binding
GO:0005515	protein binding
GO:0005524	ATP binding
GO:0008094	DNA-dependent ATPase activity
GO:0008821	crossover junction endodeoxyribonuclease activity

Biological Process

GO:0000724	double-strand break repair via homologous recombination
GO:0000737	DNA catabolic process, endonucleolytic
GO:0006200	ATP catabolic process
GO:0006259	DNA metabolic process
GO:0006281	DNA repair
GO:0006310	DNA recombination
GO:0006974	cellular response to DNA damage stimulus
GO:0010824	regulation of centrosome duplication
GO:0071140	resolution of mitotic recombination intermediates
GO:0090267	positive regulation of mitotic cell cycle spindle assembly checkpoint

Cellular Component

GO:0005634	nucleus
GO:0005657	replication fork
GO:0005737	cytoplasm
GO:0005739	mitochondrion
GO:0033065	Rad51C-XRCC3 complex
GO:0048471	perinuclear region of cytoplasm

Protein Structure and Domains

PDB ID

InterPro

IPR013632 DNA recombination and repair protein Rad51, C-terminal
IPR016467 DNA recombination and repair protein, RecA-like
IPR020588 DNA recombination and repair protein RecA-like, ATP-binding domain
IPR027417 P-loop containing nucleoside triphosphate hydrolase

PFAM

PF08423

PRINTS

PIRSF

PIRSF005856

SMART

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

O43542