InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: RTF1

Summary

InnateDB Protein

IDBP-7059.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

RTF1

Protein Name

Rtf1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae)

Synonyms

GTL7; KIAA0252;

Species

Homo sapiens

Ensembl Protein

ENSP00000374280

InnateDB Gene

IDBG-7053 (RTF1)

Protein Structure

UniProt Annotation

Function

Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non- phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1; it promotes leukemogenesis through association with KMT2A/MLL1-rearranged oncoproteins, such as KMT2A/MLL1-MLLT3/AF9 and KMT2A/MLL1-MLLT1/ENL. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. In case of infection by influenza A strain H3N2, PAF1C associates with viral NS1 protein, thereby regulating gene transcription. Binds single-stranded DNA. Required for maximal induction of heat-shock genes. Required for the trimethylation of histone H3 'Lys-4' (H3K4me3) on genes involved in stem cell pluripotency; this function is synergistic with CXXC1 indicative for an involvement of a SET1 complex (By similarity). {ECO:0000250}.

Subcellular Localization

Nucleus, nucleoplasm {ECO:0000250}.

Disease Associations

Tissue Specificity

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 37 experimentally validated interaction(s) in this database.

Experimentally validated
Total	37 [view]
Protein-Protein	34 [view]
Protein-DNA	3 [view]
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003677	DNA binding
GO:0003697	single-stranded DNA binding
GO:0005515	protein binding
GO:0044822	poly(A) RNA binding

Biological Process

GO:0000122	negative regulation of transcription from RNA polymerase II promoter
GO:0001711	endodermal cell fate commitment
GO:0006352	DNA-templated transcription, initiation
GO:0016055	Wnt signaling pathway
GO:0016570	histone modification
GO:0019827	stem cell maintenance
GO:0032968	positive regulation of transcription elongation from RNA polymerase II promoter
GO:0045944	positive regulation of transcription from RNA polymerase II promoter
GO:0051571	positive regulation of histone H3-K4 methylation
GO:0080182	histone H3-K4 trimethylation