InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: RBX1

Summary

InnateDB Protein

IDBP-8964.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

RBX1

Protein Name

ring-box 1, E3 ubiquitin protein ligase

Synonyms

BA554C12.1; RNF75; ROC1;

Species

Homo sapiens

Ensembl Protein

ENSP00000216225

InnateDB Gene

IDBG-8962 (RBX1)

Protein Structure

UniProt Annotation

Function

E3 ubiquitin ligase component of multiple cullin-RING- based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins, including proteins involved in cell cycle progression, signal transduction, transcription and transcription-coupled nucleotide excision repair. The functional specificity of the E3 ubiquitin-protein ligase complexes depends on the variable substrate recognition components. As a component of the CSA complex promotes the ubiquitination of ERCC6 resulting in proteasomal degradation. Through the RING-type zinc finger, seems to recruit the E2 ubiquitination enzyme, like CDC34, to the complex and brings it into close proximity to the substrate. Probably also stimulates CDC34 autoubiquitination. May be required for histone H3 and histone H4 ubiquitination in response to ultraviolet and for subsequent DNA repair. Promotes the neddylation of CUL1, CUL2, CUL4 and CUL4 via its interaction with UBE2M. Involved in the ubiquitination of KEAP1, ENC1 and KLHL41. In concert with ATF2 and CUL3, promotes degradation of KAT5 thereby attenuating its ability to acetylate and activate ATM. {ECO:0000269PubMed:10579999, ECO:0000269PubMed:11027288, ECO:0000269PubMed:15983046, ECO:0000269PubMed:16678110, ECO:0000269PubMed:16751180, ECO:0000269PubMed:18397884, ECO:0000269PubMed:19112177, ECO:0000269PubMed:19679664}.

Subcellular Localization

Cytoplasm {ECO:0000269PubMed:11027288}. Nucleus {ECO:0000269PubMed:11027288}.

Disease Associations

Tissue Specificity

Widely expressed.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 213 experimentally validated interaction(s) in this database.
They are also associated with 13 interaction(s) predicted by orthology.

Experimentally validated
Total	213 [view]
Protein-Protein	213 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	13 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0004842	ubiquitin-protein transferase activity
GO:0005515	protein binding
GO:0008270	zinc ion binding
GO:0019788	NEDD8 ligase activity
GO:0031625	ubiquitin protein ligase binding
GO:0032403	protein complex binding

Biological Process

GO:0006281	DNA repair
GO:0006513	protein monoubiquitination
GO:0007219	Notch signaling pathway
GO:0016032	viral process
GO:0016567	protein ubiquitination
GO:0030163	protein catabolic process
GO:0031146	SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
GO:0043161	proteasome-mediated ubiquitin-dependent protein catabolic process
GO:0045116	protein neddylation
GO:0061418	regulation of transcription from RNA polymerase II promoter in response to hypoxia
GO:0071456	cellular response to hypoxia

Cellular Component

GO:0005654	nucleoplasm
GO:0005829	cytosol
GO:0019005	SCF ubiquitin ligase complex
GO:0030891	VCB complex
GO:0031461	cullin-RING ubiquitin ligase complex
GO:0031462	Cul2-RING ubiquitin ligase complex
GO:0031463	Cul3-RING ubiquitin ligase complex
GO:0031464	Cul4A-RING E3 ubiquitin ligase complex
GO:0031465	Cul4B-RING E3 ubiquitin ligase complex
GO:0031466	Cul5-RING ubiquitin ligase complex
GO:0031467	Cul7-RING ubiquitin ligase complex