InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: USP14

Summary

InnateDB Protein

IDBP-261.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

USP14

Protein Name

ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase)

Synonyms

TGT;

Species

Homo sapiens

Ensembl Protein

ENSP00000261601

InnateDB Gene

IDBG-259 (USP14)

Protein Structure

UniProt Annotation

Function

Proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins. Ensures the regeneration of ubiquitin at the proteasome. Is a reversibly associated subunit of the proteasome and a large fraction of proteasome-free protein exists within the cell. Required for the degradation of the chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis. Serves also as a physiological inhibitor of endoplasmic reticulum-associated degradation (ERAD) under the non-stressed condition by inhibiting the degradation of unfolded endoplasmic reticulum proteins via interaction with ERN1. Indispensable for synaptic development and function at neuromuscular junctions (NMJs). {ECO:0000269PubMed:18162577, ECO:0000269PubMed:19106094, ECO:0000269PubMed:19135427}.

Subcellular Localization

Cytoplasm {ECO:0000269PubMed:19106094}. Cell membrane {ECO:0000269PubMed:19106094}; Peripheral membrane protein {ECO:0000269PubMed:19106094}.

Disease Associations

Tissue Specificity

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 70 experimentally validated interaction(s) in this database.
They are also associated with 4 interaction(s) predicted by orthology.

Experimentally validated
Total	70 [view]
Protein-Protein	70 [view]
Protein-DNA	0
Protein-RNA	0
DNA-DNA	0
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	4 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0004197	cysteine-type endopeptidase activity
GO:0004221	ubiquitin thiolesterase activity
GO:0004843	ubiquitin-specific protease activity
GO:0004866	endopeptidase inhibitor activity
GO:0005515	protein binding
GO:0008193	tRNA guanylyltransferase activity
GO:0036459	ubiquitinyl hydrolase activity
GO:0070628	proteasome binding

Biological Process

GO:0006511	ubiquitin-dependent protein catabolic process
GO:0007268	synaptic transmission
GO:0010951	negative regulation of endopeptidase activity
GO:0016579	protein deubiquitination
GO:0050920	regulation of chemotaxis
GO:0061136	regulation of proteasomal protein catabolic process

Cellular Component

GO:0000502	proteasome complex
GO:0005737	cytoplasm
GO:0005886	plasma membrane
GO:0009986	cell surface
GO:0016023	cytoplasmic membrane-bounded vesicle
GO:0045202	synapse

Protein Structure and Domains

PDB ID

InterPro

IPR000626 Ubiquitin-like
IPR001394 Peptidase C19, ubiquitin carboxyl-terminal hydrolase
IPR028889 Ubiquitin carboxyl-terminal hydrolase-like domain
IPR029071 Ubiquitin-related domain

PFAM

PF00240
PF14560
PF00443

PRINTS

PIRSF

SMART

SM00213

TIGRFAMs

Post-translational Modifications

Modification

Cross-References

SwissProt

P54578