InnateDB: Systems Biology of the Innate Immune Response

Homo sapiens Protein: PIAS1

Summary

InnateDB Protein

IDBP-18828.6

Last Modified

2014-10-13 [Report errors or provide feedback]

Gene Symbol

PIAS1

Protein Name

protein inhibitor of activated STAT, 1

Synonyms

DDXBP1; GBP; GU/RH-II; ZMIZ3;

Species

Homo sapiens

Ensembl Protein

ENSP00000249636

InnateDB Gene

IDBG-18826 (PIAS1)

Protein Structure

UniProt Annotation

Function

Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. In vitro, binds A/T-rich DNA. The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context. Together with PRMT1, may repress STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling. Sumoylates PML (at'Lys-65' and 'Lys-160') and PML-RAR and promotes their ubiquitin-mediated degradation. PIAS1-mediated sumoylation of PML promotes its interaction with CSNK2A1/CK2 which in turn promotes PML phosphorylation and degradation (By similarity). Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation. {ECO:0000250, ECO:0000269PubMed:14500712, ECO:0000269PubMed:19136629, ECO:0000269PubMed:21965678}.

Subcellular Localization

Nucleus speckle {ECO:0000269PubMed:12393906, ECO:0000269PubMed:19136629, ECO:0000269PubMed:9177271}. Nucleus, PML body {ECO:0000250}. Note=Interaction with CSRP2 may induce a partial redistribution along the cytoskeleton.

Disease Associations

Tissue Specificity

Expressed in numerous tissues with highest level in testis. {ECO:0000269PubMed:11439351, ECO:0000269PubMed:9177271}.

Comments

Interactions

Number of Interactions

This gene and/or its encoded proteins are associated with 143 experimentally validated interaction(s) in this database.
They are also associated with 15 interaction(s) predicted by orthology.

Experimentally validated
Total	143 [view]
Protein-Protein	135 [view]
Protein-DNA	6 [view]
Protein-RNA	0
DNA-DNA	2 [view]
RNA-RNA	0
DNA-RNA	0

Predicted by orthology
Total	15 [view]

Gene Ontology

Molecular Function

Accession	GO Term
GO:0003677	DNA binding
GO:0003713	transcription coactivator activity
GO:0003714	transcription corepressor activity
GO:0005515	protein binding
GO:0008270	zinc ion binding
GO:0019789	SUMO ligase activity
GO:0019899	enzyme binding
GO:0019904	protein domain specific binding
GO:0031625	ubiquitin protein ligase binding
GO:0050681	androgen receptor binding

Biological Process

GO:0000122	negative regulation of transcription from RNA polymerase II promoter
GO:0006351	transcription, DNA-templated
GO:0007259	JAK-STAT cascade
GO:0007283	spermatogenesis
GO:0016925	protein sumoylation
GO:0019221	cytokine-mediated signaling pathway
GO:0030521	androgen receptor signaling pathway
GO:0032436	positive regulation of proteasomal ubiquitin-dependent protein catabolic process
GO:0033235	positive regulation of protein sumoylation
GO:0042127	regulation of cell proliferation
GO:0045087	innate immune response (InnateDB)
GO:0045893	positive regulation of transcription, DNA-templated
GO:0051152	positive regulation of smooth muscle cell differentiation
GO:0060333	interferon-gamma-mediated signaling pathway
GO:0060334	regulation of interferon-gamma-mediated signaling pathway
GO:0065004	protein-DNA complex assembly